Abstract DNA replication is essential for the proliferation of all cells.Bacterial chromosomes are replicated bidirectionally from a single read more origin of replication, with replication proceeding at about 1000 bp per second.For the model organism, Escherichia coli, this translates into a replication time of about 40 min for its 4.6 Mb chromoso
Simple non-fluorescent polarity labeling of microtubules for molecular motor assays
Transport of intracellular organelles along the microtubule cytoskeleton occurs in a bidirectional manner due to opposing activity of microtubule-associated motor proteins of the kinesin and dynein families.Regulation of this opposing activity and the resultant motion is believed to generate a polarized distribution of many organelles within the ce
A novel mechanical design of disc brakes for fault diagnosis and monitoring positive braking pressure in mine hoist
As the final executor of the braking system of coal mine hoists, the disc brake directly affects the safety and reliability of mine hoists.Aimed to the problem of failure of hoist disc brake, according to its structural characteristics and functional requirements, this article proposes a disc brake that monitors positive brake pressure in real time